ISSN 2394-5125
 

Research Article 


ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS

Archana Kulkarni, Anuradha Deo, Seema Nimbarte.

Abstract
The emergence of resistance to antimicrobial agents is a global public health problem, particularly
in pathogens causing nosocomial infections. The most common nosocomial pathogens include Staphylococci
(especially Staphylococcus aureus), Pseudomonas, Escherichia coli, Klebsiella and Proteus. Concerns about
resistance have led to not only the correct and judicious use of antibiotics but also more stringent infection
control measures to reduce the transmission of infection because the organisms that cause nosocomial infections
are often drug-resistant.. One such measure encouraged is the use of different disinfectants. QACs (Quaternary
ammonium compounds) are the most useful antiseptics and disinfectants used in hospitals. Cetrimide is one such
strong monocationic surfactant, a QAC. In the present study >50% S.aureus isolates collected from hospital
environment showed resistance to antibiotics, such as teicoplanin(100%) erythromycin(50%), tetracycline (60%)
cephalothin(40%), methicillin (40%), rifampicin(40%) and co-trimoxazole (10%). The MIC of cetrimide against
these resistant Staphylococcus aureus varied between 9.765 and 312.5 mcg/ml with maximum number of
isolates (30 %) showing the MIC value of 312.5 mcg/ml. The bactericidal activity of cetrimide against S.aureus
evaluated by time kill assay indicated a complete killing of bacteria after 30min. Transmission electron
microscopic studies on the effect of cetrimide on methicillin resistant Staphylococcus aureus revealed that the
nuclear areas become obscured by an inflow of the cytoplasm, followed by loss of granularity of cytoplasm,
disintegration of cell wall, cell membrane and condensation of cytoplasm, loss of cell constituents and break
down of cell wall and extensive damage to cytoplasmic membrane and ultimately cell lysis. The ultra structural
studies showed that antibiotic resistance does not alter the susceptibility of antibiotic resistant S.aureus to QAC
cetrimide.

Key words: QAC, cetrimide, S.aureus, time kill assay, bactericidal.


 
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How to Cite this Article
Pubmed Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. JCR. 2020; 7(16): 1053-1065. doi:10.31838/jcr.07.16.135


Web Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. http://www.jcreview.com/?mno=4673 [Access: May 30, 2021]. doi:10.31838/jcr.07.16.135


AMA (American Medical Association) Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. JCR. 2020; 7(16): 1053-1065. doi:10.31838/jcr.07.16.135



Vancouver/ICMJE Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. JCR. (2020), [cited May 30, 2021]; 7(16): 1053-1065. doi:10.31838/jcr.07.16.135



Harvard Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte (2020) ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. JCR, 7 (16), 1053-1065. doi:10.31838/jcr.07.16.135



Turabian Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. 2020. ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. Journal of Critical Reviews, 7 (16), 1053-1065. doi:10.31838/jcr.07.16.135



Chicago Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. "ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS." Journal of Critical Reviews 7 (2020), 1053-1065. doi:10.31838/jcr.07.16.135



MLA (The Modern Language Association) Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte. "ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS." Journal of Critical Reviews 7.16 (2020), 1053-1065. Print. doi:10.31838/jcr.07.16.135



APA (American Psychological Association) Style

Archana Kulkarni, Anuradha Deo, Seema Nimbarte (2020) ANTIBIOTIC RESILIENCE PATTERN AND CETRIMIDE INDUCED ULTRA STRUCTURAL CHANGES IN MULTIDRUG RESISTANT S. AUREUS. Journal of Critical Reviews, 7 (16), 1053-1065. doi:10.31838/jcr.07.16.135