ISSN 2394-5125
 

Research Article 


INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA.

Abstract
Background: Multi genetic alterations lead to cancer progression, it is seldom possible to treat with
monotherapy. Thus, nowadays, clinicians are using combinational drug therapy for cancer. As a synthetic drug
treatment displays several adverse effects, the combination of natural products with synthetic drug therapy
gained its importance.
Methodology: In this current study, we investigated the combination anticancer effect of Doxorubicin,
Hispolon, and D-Limonene against colorectal cancer cell lines was determined by MTT assay. The antioxidant
enzyme activity of various combinations of tri-drug on COLO-205 and HCT-116 cells was performed. The
activity of antioxidant enzymes, SOD, Catalase, Gpx and GST was measured. The free radical scavenging
activity of DOX, HIS and D-LIM was performed on DPPH, ABTS and nitric oxide free radicals measured. The
gene expression levels of NF-κB P65 and BAX upon induction of DOX, HIS and D-LIM to the COLO 205 &
HCT 116 cells was determined by Western blotting method.
Results: The cell viability, enzymatic, time and dose-dependent cytotoxicity assays demonstrated the anticancer
effect of our tri drug-combination against COLO-205, HCT-116 cell lines. Besides, western blot results of tri
drug-combination treatment displayed significantly decreased the levels of NF-κB P65.
Conclusion: Therefore, the current study results revealed that, the tri drug-combination of DOX, HIS and DLIM
treatment beneficial in colon cancer therapy.

Key words: Doxorubicin, Hispolon, D-Limonene, NF-κB P65, combination, synergy, colon cancer, Apoptosis, COLO-205, HCT-116


 
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How to Cite this Article
Pubmed Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. JCR. 2020; 7(4): 2608-2615. doi:10.31838/jcr.07.04.399


Web Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. http://www.jcreview.com/?mno=134802 [Access: May 30, 2021]. doi:10.31838/jcr.07.04.399


AMA (American Medical Association) Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. JCR. 2020; 7(4): 2608-2615. doi:10.31838/jcr.07.04.399



Vancouver/ICMJE Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. JCR. (2020), [cited May 30, 2021]; 7(4): 2608-2615. doi:10.31838/jcr.07.04.399



Harvard Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA (2020) INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. JCR, 7 (4), 2608-2615. doi:10.31838/jcr.07.04.399



Turabian Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. 2020. INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. Journal of Critical Reviews, 7 (4), 2608-2615. doi:10.31838/jcr.07.04.399



Chicago Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. "INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS." Journal of Critical Reviews 7 (2020), 2608-2615. doi:10.31838/jcr.07.04.399



MLA (The Modern Language Association) Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA. "INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS." Journal of Critical Reviews 7.4 (2020), 2608-2615. Print. doi:10.31838/jcr.07.04.399



APA (American Psychological Association) Style

CHANDRA SEKHAR K, MANNUR ISMAIL SHAIK, MD. NAZNEEN BOBBY, JAGADEESWARA REDDY KANALA (2020) INVESTIGATION ON SYNERGISTIC EFFECT OF DOXORUBICIN IN COMBINATION WITH HISPOLON AND D-LIMONENE AGAINST HUMAN COLON CANCER CELLS. Journal of Critical Reviews, 7 (4), 2608-2615. doi:10.31838/jcr.07.04.399