ISSN 2394-5125
 

Review Article 


THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E.

Abstract
42 native yeast strains isolated from 5 wine grapes of the Tehran Center for Fruits and Vegetables were studied, among which 6 strains were selected according to the results of tests for resistance to high concentrations of ethanol and glucose; according to the results of screening for the amount of ethanol produced, one strain was left in the study, fermenting the maximum amount of alcohol in a given culture medium 16%. Strain number Af was a new strain of yeast, identified in another study and named by the author of this article as Hanseniaspora Opuntiae MK 460485. Quantitative chromatographic evaluation of ethanol production by Hanseniaspora opuntiae MK 460485 strain was carried out for various carbon sources (glucose, sucrose, fructose syrup, malt, molasses), which showed that the highest amount of ethanol is fermented with yeast in the presence of molasses (17%). strain with various sources of nitrogen (ammonium phosphate, ammonium sulfate, urea, ammonium nitrate, peptone and ammonium chloride) showed that the best production of alcohol goes to culture media x to ammonium nitrate (17.3%). The main factors for ethanol fermentation were screened and optimized based on the mathematical method of experiment planning using the Minitab© program and the Plackett-Burman package. It was shown that the main factors in ethanol production by this strain are sources of carbon, nitrogen, and temperatures, and pH does not have a significant effect. A mathematical model is obtained, based on which the optimal conditions for the cultivation of the strain are revealed: molasses concentration 56.5% ammonium nitrate 3 g/l; cultivation temperature 30.4 °C. Ethanol production under optimal conditions increased upto 19.6%, that is considerable result and investigation in the area of ethanol production.

Key words: Yeast, Ethanol, Carbon, Nitrogen, Hanseniaspora opuntiae MK 460485.


 
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Pubmed Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. JCR. 2020; 7(4): 498-505. doi:10.31838/jcr.07.04.94


Web Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. http://www.jcreview.com/?mno=94813 [Access: March 24, 2020]. doi:10.31838/jcr.07.04.94


AMA (American Medical Association) Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. JCR. 2020; 7(4): 498-505. doi:10.31838/jcr.07.04.94



Vancouver/ICMJE Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. JCR. (2020), [cited March 24, 2020]; 7(4): 498-505. doi:10.31838/jcr.07.04.94



Harvard Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E (2020) THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. JCR, 7 (4), 498-505. doi:10.31838/jcr.07.04.94



Turabian Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. 2020. THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. Journal of Critical Reviews, 7 (4), 498-505. doi:10.31838/jcr.07.04.94



Chicago Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. "THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS." Journal of Critical Reviews 7 (2020), 498-505. doi:10.31838/jcr.07.04.94



MLA (The Modern Language Association) Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E. "THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS." Journal of Critical Reviews 7.4 (2020), 498-505. Print. doi:10.31838/jcr.07.04.94



APA (American Psychological Association) Style

Mezenova. O.Ya, Keshtkar.S, Kulaev K.T, Danshina. S.D, Romiani.E (2020) THE STUDY OF ETHANOL PRODUCTION BY NEW STRAIN OF YEASTS "HANSENIASPORA OPUNTIAE MK 460485", INVESTIGATION OF ITS ETHANOL PRODUCTION IN PRESENCE OF DIFFERENT CARBON AND NITROGEN SOURCES, AND OPTIMAL CONDITIONS. Journal of Critical Reviews, 7 (4), 498-505. doi:10.31838/jcr.07.04.94