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2021, Vol: 8, Issue: 2
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Research Article 


Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar.

Abstract
Background: Blastocystis hominis is a common intestinal protozoan with a prevalence reported variously from
different countries. Considering the relatively high prevalence of this condition and the emphasis on its pathogenesis,
this study determined Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for
Detection of Blastocystis on health centers in Kerman, Iran
Methods: A total of 210 single samples of stool were collected from patients presenting to healthcare centers in
Kerman. The samples were transferred to laboratory in the same day and examined for diagnosis of infection with
blastocystis hominis using direct method, concentrating method (formalin-ether), Trichrome staining method
(trichrome), and Real-time PCR molecular method.
Results: The frequency of blastocystis hominis using direct method and Trichrome staining method showed that 30
cases under study (%14.3) were positive including 17 females and 13 males. In the molecular method, 66 cases
(%31.42) were positive for infection with blastocystis hominis including 30 (%45.5) men and 36 (%54.5) women. In
this study, the maximum and minimum frequencies pertained to genotype (subtype) 3 (%56.06) and genotype 2
(%1.51) with no cases found for genotype 6. Also, mixed infection with genotypes 3 and 4 (%42.88), genotypes 4 and
5 (%14.28), genotypes 3 and 5 (%14.28), genotypes 1, 2 and 3 (%14.28), genotypes 3, 4, and 7 (%14.28) were
reported.
Conclusion: Hence, promoting the knowledge about personal improving environmental sanitation, and supplying
healthful drinking water can prove effective in decreasing infection with blastocystis hominis.
Blastocystis hominis, direct method, trichrome staining method, molecular method.

Key words: Blastocystis hominis, direct method, trichrome staining method, molecular method.


 
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How to Cite this Article
Pubmed Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. JCR. 2021; 8(1): 10-16. doi:10.31838/jcr.08.02.2

Web Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. http://www.jcreview.com/?mno=39287 [Access: April 10, 2021]. doi:10.31838/jcr.08.02.2

AMA (American Medical Association) Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. JCR. 2021; 8(1): 10-16. doi:10.31838/jcr.08.02.2


Vancouver/ICMJE Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. JCR. (2021), [cited April 10, 2021]; 8(1): 10-16. doi:10.31838/jcr.08.02.2


Harvard Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar (2021) Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. JCR, 8 (1), 10-16. doi:10.31838/jcr.08.02.2


Turabian Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. 2021. Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. Journal of Critical Reviews, 8 (1), 10-16. doi:10.31838/jcr.08.02.2


Chicago Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. "Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran." Journal of Critical Reviews 8 (2021), 10-16. doi:10.31838/jcr.08.02.2


MLA (The Modern Language Association) Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar. "Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran." Journal of Critical Reviews 8.1 (2021), 10-16. Print. doi:10.31838/jcr.08.02.2


APA (American Psychological Association) Style

Ali Fattahi Bafghi, Rafat Hosseini, Hamid Reza Mollaei, Kazem Barzegar (2021) Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman, Iran. Journal of Critical Reviews, 8 (1), 10-16. doi:10.31838/jcr.08.02.2


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